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human skin fibroblasts hs 27 cell line  (ATCC)


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    ATCC human skin fibroblasts hs 27 cell line
    Human Skin Fibroblasts Hs 27 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 737 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 737 article reviews
    human skin fibroblasts hs 27 cell line - by Bioz Stars, 2026-02
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    Normal Human Skin Fibroblast Hbf4 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    A. WS-1 human fibroblast viability for indicated clemastine concentrations and timepoints. B-C. Representative Immunofluorescence micrographs of control untreated (B, N=10) and clemastine treated (2 µg/mL, 18 h, N=10) (C) WS-1 human fibroblasts. Lysosomal LAMP2 (red) and LGALS1 (green) are visualized. LAMP2 + /LGALS1 + permeable lysosomes are identified by arrows. Cell nuclei counterstained with DAPI (blue). Scalebar: 10 µm. D. Clemastine DSS, AUC and EC50 scores in three fibroblast lines (hPSC, WS1, CAF82) in their starved and non-starved states. E. Table of putative myCAF/iCAF marker genes and the percentages of cells positive for these in the starved and non-starved HPSC cultures. Average (avg) log2 fold change (FC) for the transcription level differences in activated iCAF-like cells versus parental HPSCs. Adjusted p-values. The change in transcription level (avg log2FC) ranges from blue (downregulated) to orange (upregulated). F. Violin plots of scRNAseq gene expression levels of CAF markers vimentin, FAP, and ACTA2 (gene coding for the α-SMA protein) in parental, non-starved (red) and starved, iCAF-like transformed, hPSCs (turquoise). G. Representative micrographs of FAP, vimentin, and α-SMA in non-starved (red) and starved (turquoise) HPSCs visualized by IF staining. H . Violin plots of scRNAseq gene expression levels of lysosomal markers in non-starved (red) and starved (turquoise) HPSCs. I-J . IF staining of LGALS1 (green) and LAMP2 (red) in untreated (DMSO) and clemastine-treated non-starved (I) and starved (J) HPSCs. LAMP2 + /LGALS1 + permeable lysosomes are identified by arrows. Clemastine treatment causes cytoplasmic LGALS1 to relocate to damaged lysosomes. Clemastine treatment also upregulated LAMP2 in HPSCs, but not in iCAF-like cells. LGALS1 relocation is also observed in untreated iCAF-like cells. *p<0.05, **p<0.001, ***p<0.0001.

    Journal: bioRxiv

    Article Title: Therapeutic Eradication of Cancer-associated Fibroblasts Inhibits in vivo progression of Pancreatic Cancer

    doi: 10.1101/2025.11.04.686484

    Figure Lengend Snippet: A. WS-1 human fibroblast viability for indicated clemastine concentrations and timepoints. B-C. Representative Immunofluorescence micrographs of control untreated (B, N=10) and clemastine treated (2 µg/mL, 18 h, N=10) (C) WS-1 human fibroblasts. Lysosomal LAMP2 (red) and LGALS1 (green) are visualized. LAMP2 + /LGALS1 + permeable lysosomes are identified by arrows. Cell nuclei counterstained with DAPI (blue). Scalebar: 10 µm. D. Clemastine DSS, AUC and EC50 scores in three fibroblast lines (hPSC, WS1, CAF82) in their starved and non-starved states. E. Table of putative myCAF/iCAF marker genes and the percentages of cells positive for these in the starved and non-starved HPSC cultures. Average (avg) log2 fold change (FC) for the transcription level differences in activated iCAF-like cells versus parental HPSCs. Adjusted p-values. The change in transcription level (avg log2FC) ranges from blue (downregulated) to orange (upregulated). F. Violin plots of scRNAseq gene expression levels of CAF markers vimentin, FAP, and ACTA2 (gene coding for the α-SMA protein) in parental, non-starved (red) and starved, iCAF-like transformed, hPSCs (turquoise). G. Representative micrographs of FAP, vimentin, and α-SMA in non-starved (red) and starved (turquoise) HPSCs visualized by IF staining. H . Violin plots of scRNAseq gene expression levels of lysosomal markers in non-starved (red) and starved (turquoise) HPSCs. I-J . IF staining of LGALS1 (green) and LAMP2 (red) in untreated (DMSO) and clemastine-treated non-starved (I) and starved (J) HPSCs. LAMP2 + /LGALS1 + permeable lysosomes are identified by arrows. Clemastine treatment causes cytoplasmic LGALS1 to relocate to damaged lysosomes. Clemastine treatment also upregulated LAMP2 in HPSCs, but not in iCAF-like cells. LGALS1 relocation is also observed in untreated iCAF-like cells. *p<0.05, **p<0.001, ***p<0.0001.

    Article Snippet: The human skin fibroblast cell line WS1 (ATCC) was cultured according to the provider’s instructions.

    Techniques: Immunofluorescence, Control, Marker, Gene Expression, Transformation Assay, Staining